Ancestral-specific reference genomes and uses thereof

ABSTRACT

Ancestry has a significant impact on the major and minor alleles found in each nucleotide position within the genome. Due to mechanisms of inheritance, ancestral-specific information contained within the genome is conserved within members of an ancestry. For this reason, individuals within a specific ancestry are more likely to share alleles in their genomes with other members of the same ancestry. Functionally, the combination of alleles at all positions within a group of individuals defines that group as having a common ancestry. Moreover, the aggregation of differences between alleles at all positions distinguishes one ancestry from another. The genomic similarities and differences between ancestries provides a mechanism to generate reference genomes that are specific for each ancestry. Reference genomes that are specific to an ancestry can be used to increase the accuracy of whole genome sequencing, DNA-based diagnostics and therapeutic marker discovery and in a variety of real-,world DNA-based applications. Provided herein are methods for diagnosis with an ancestral-specific reference genome.

1. CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of U.S. Ser. No. 15/249,409, filed on Aug. 27, 2016, which is a continuation of U.S. Ser. No. 13/834,685, filed on Mar. 15, 2013, now U.S. Pat. No. 9,449,143, issued Sep. 20, 2016, and claims the benefit of U.S. Provisional Patent Application Ser. No. 61/694,155, filed on Aug. 28, 2012 each of which are incorporated herein by reference in their entirety.

2. FIELD OF THE INVENTION

Provided herein are ancestral-specific.reference genome databases and methods of making and using such ancestral-specific reference genome databases.

3. BACKGROUND OF THE INVENTION

Much progress has been made in the development of high-throughput DNA sequencing technology in recent years (Pettersson E, Lundeberg J, Ahmadian A (February 2009). “Generations of sequencing technologies”. Genomics 93 (2): 105-11. doi:10.1016/j.ygeno.2008.10.003. PMID 18992322; Staden, R (1979 Jun. 11). “A strategy of DNA sequencing employing computer programs”. Nucleic Acids Research 6 (7): 2601-10. doi:10.1093/nar/6.7.2601. PMID 461197; Church G M (January 2006). “Genomes for all”. Sci. Am. 294 (1): 46-54. doi:10.1038/scientificamerican0106-46. PMID 16468433). However, a comprehensive analysis of the entire genome is not currently commercially available or technologically possible. To date, whole genome sequencing is used only for research purposes (completegenomics.com/services/standard-sequencing/; illumina.com/services.ilmn), and a medically useful whole-genome-sequencing scale service simply does not exist.

While there are some reports of whole-genome-medical sequencing services, such services utilize information from the whole genome for only a few disease-associated single nucleotide polymorphisms (SNPs) in a limited number of genes (illumina.com/services.ilmn). This is in part because, although ancestral-specific mutations useful for medical applications of whole-genome sequencing have been generated in a variety of diseases (ncbi.nlm.nih.gov/omim), and Genome Wide Association Studies (GWAS) (Klein R J, Zeiss C, Chew E Y, Tsai J Y, Sackler R S, Haynes C, Henning A K, SanGiovanni J P, Mane S M, Mayne S T, Bracken M B, Ferris F L, Ott J, Barnstable C, Holt J (April 2005). “Complement Factor H Polymorphism in Age-Related Macular Degeneration”. Science 308 (5720): 385-9. doi:10.1126/science.1109557. PMC 1512523. PMID 15761122) has generated a partial list of ancestral SNPs for research purposes, a comprehensive list of whole genome-wide ancestral SNPs has not been generated to date. Without a comprehensive list of ancestral SNPs, the development of whole genome sequencing as a medical diagnostic tool may not be possible

Progress in the area of whole genome sequencing as an approved diagnostic tool has been impeded largely because medical sequencing methods developed to date generate a large number of false positive and false negative base calls inherent to the technology (Zhao J, Grant S F (February 2011). “Advances in Whole. Genome Sequencing Technology”. Curr Pharm Biotechnol 23(2) 293-305. PMID 21050163). There is an additional layer of misinformation generated in whole genome sequencing due to the current NIH-derived reference genome used as the:standard template for sequencing (Scherer, Stewart (2008). A short guide to the human genome. CSHL Press. p. 135. ISBN 0-87969-791-1; Wheeler D A, Srinivasan M, Egholm M, Shen Y, Chen L, McGuire A, He W, Chen Y J, Makhijani V, Roth G T, Gomes X, Tartaro K, Niazi F, Turcotte C L, Irzyk G P, Lupski J R, Chinault C, Song X Z, Liu Y, Yuan Y, Nazareth L, Qin X, Muzny D M, Margulies M, Weinstock G M, Gibbs R A, Rothberg J M. (2008). “The complete genome of an individual by massively parallel DNA sequencing”. Nature 452 (7189): 872-6; Bibcode 2008 Natur. 452 . . . 872W. doi:10.1038/nature06884. PMID 18421352). In particular, all of the existing sequencing technologies utilize the same standard reference genome for the bioinformatic reconstruction/assembly of the whole genome from the small DNA fragments and sequenced during the process of obtaining a medically usable completed whole genome. The current standard reference genome, which was generated some years ago by the National Institutes of Health (NIH) as a model for genomic structure and sequence assembly, is based on a single whole genome sequence generated from the composite DNA obtained originally from five different individuals (Editorial (October 2010). “E pluribus unum”. Nature Methods. 331 (5): 331. doi:10.1038/nmeth0510-331). As such, it is neither statistically significant nor accurate when comparing individuals from different ancestral backgrounds and may not provide a statistically significant reference for interpreting genomic,inforrnation, especially for clinical diagnostics and pharmaceutical development.

Although some sequencing companies claim to have a very high accuracy rate for determining a whole genome sequence. (Quail, Michael; Smith, Miriam E; Coupland, Paul; Otto, Thomas D; Harris, Simon R; Connor, Thomas R; Bertoni, Anna; Swerdlow, Harold P; Gu, Yong (1 Jan. 2012). “A tale of three next generation sequencing platforms: comparison of Ion torrent, pacific biosciences and illumina MiSeq sequencers”. BMC Genomics 13 (1): 341. doi:10.1186/1471-2164-13-341; Liu, Lin; Li, Yinhu; Li, Siliang; Hu, Ni; He, Yimin; Pong, Ray; Lin, Danni; Lu, Lihua; Law, Maggie (1 Jan. 2012). “Comparison of Next-Generation Sequencing Systems”. Journal of Biomedicine and Biotechnology 2012: 1-11. doi:10.1155/2012/251364), the reality is, due to the large size of the genome (˜3.2 billion base pairs coding for 20,000 to 25,000 distinct genes), even a small percentage of errors results in a large number of bases that are incorrectly called. A very low error rate is required for predictive medicine applications (Bentley D R (December 2006). “Whole-genome re-sequencing”. Curr. Opin. Genet. Dev. 16 (6): 545-552. doi:10.1016/j.gde.2006.10.009. PMID 17055251; Genetest.org). Recently, bioinformatic tools have been developed that validate genomic sequence based on familial sequence information for an individual family (farnilygenornics.systemsbiology.net/publications). Including familial information from three closely related individuals can improve DNA sequence accuracy by 70%. Using information from four or more family members increases accuracy by 90% (Roach J C, GlussmanG, Smit A F, Huff C D, Drmanac R, Jorde L B, Hood L, Galas D J (10 Apr. 2010) “Analysis of Genetic Inheritance in a Family Quartet by Whole Genome. Sequencing”. Science 328: 636-9 doi:10.3410/f.2707961.2371060). However, such correction tools are time-consuming and add inefficiency and cost to the process of whole genome sequencing.

Accordingly, there is a need for the development of an ancestral-specific reference genome database that incorporates familial genome sequencing information to improve the accuracy of such ancestral-specific reference genomes. An ancestral-specific reference databases can, in tum, be used as tool, for example, for the diagnosis of a patent at risk for a genetic disease or disorder or for the prognosis of such a genetic disease or disorder.

4. SUMMARY OF THE INVENTION

Advantageously, the present disclosure provides for the construction of ancestral-specific reference genomes, genome databases, ancestral-specific genomes, and methods of use that overcome the deficiencies present in the art. Specifically, the present disclosure provides ancestral-specific genomes, databases, and uses thereof, where such genomes include whole genomes, partial genomes, epigenomes, and exome sequences. Such ancestral-specific genomes and methods for use provided by the present disclosure have higher accuracy and eliminate the false-positive problem inherent in previous methods. The accuracy of properly identifying a variant as disease-causing, familial or ancestral depends, in part, on the ability to determine if the variant is novel. Accordingly, using, the methods disclosed herein, the number of novel SNPs identified is greater than 175,000, greater than 200,000, greater than 250,000, greater than 300,000, greater than 350,000, or greater than 400,000. As such, the methods disclosed herein, at least double the number of novel SNPs identified relative to prior art methods. The increase in novel SNPs identified increases the mean mapped depth of DNA sequencing. Using the methods disclosed herein, the mean mapped depth is between about 20 and about 60. For example, the mean mapped depth is between about 30 and about 60, between about 40 and about 60. Additionally, the mean mapped depth is greater than 20, greater than 30, greater than 40, greater than 50, or greater than 60. This mean mapped depth is significantly greater than previous methods which achieve a mean mapped depth of about 5.1 or about 7. Accordingly, the methods disclosed herein provide a significantly greater mean mapped depth relative to prior art methods. The increased mean mapped depth and increase in novel SNPs identified minimizes erroneous variant calls and results in greater accuracy in the annotation of, ancestral-specific variants. The databases and method provided herein have a wide variety of applications including use in humans, animals, microorganisms, and plants.

In one aspect, a method of using an ancestral-specific reference genome is provided, where the ancestral-specific reference genome constructed by steps comprising: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome, data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genorne sequences with the same ancestry; and (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference genome.

In a second aspect, the present disclosure provides for a method of determining a partial ancestral-specific reference genome constructed by steps comprising: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; and (h) comparing a DNA sequence of the subject's partial genome with the ancestral-specific reference partial genome to determine the partial ancestral-specific genome of the subject.

In a further aspect, the disclosure provides for a method of determining a whole or partial ancestral-specific reference exome sequence constructed by the steps comprising: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating, steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference whole genome to determine the coding regions of the genome; and i) using the coding regions of the genome identified to construct a whole or partial ancestral-specific exome sequence of the subject.

In an addition aspect, the present disclosure provides for a method for diagnosing a subject using an ancestral specific reference database comprising the steps of: (a) obtaining a subject's DNA; (b)sequencing the subject's DNA to assemble the subject's whole or partial genome or whole or partial exome ; (c) during or prior to assembly of the subject's whole or partial genome or exome, annotating the subject's whole or partial genome or whole or partial exome based on the ancestral-specific reference,database; and (d) identifying one or more genome sequence or exome sequence in the subject that differs from the one or more genome sequence or exome sequence in the ancestral-specific reference genome thereby identifying mutations or disease-causing variants in the one or more whole or partial genome sequence or whole or partial exome sequence of the subject's DNA to diagnose the subject.

In another aspect, the disclosure provides for a method of constructing one or more ancestral-specific sequence arrays, constructed by the steps comprising.: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference whole genome to determine the coding regions of the genome; i) determining the genomic or exomic information used to identify ancestral patterns; and j) generating one or more sequence array(s) that is limited to ancestral-specific genomic or exomic information.

In a further aspect, the disclosure provides for a method for annotating a genome of a subject. The method comprises: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; and (h) annotating the subject's genome using the ancestral-specific reference genome, where such an annotation step can be performed before step a), at any point during steps a)-h) and after step h).

In another aspect, the disclosure provides a method for annotating a genome of a subject, where the steps of the method preferably comprise (a) obtaining a subject's DNA; (b)sequencing the subject's DNA to assemble the subject's whole or partial genome or whole or partial exome ; (c) during or prior to assembly of the subject's whole or partial genome or exome, annotating the subject's whole or partial genome or whole or partial exome based on the ancestral-specific reference database; and (d) identifying one or more genome sequence or exome sequence in the subject that differs from the one or more genome sequence or exome sequence in the ancestral-specific reference genome thereby identifying mutations or disease-causing variants in the one or more whole or partial genome sequence or whole or partial exome sequence of the subject's DNA to diagnose the subject.

In a further aspect, the disclosure provides for a method for identifying one or more genome sequence or exome sequence that are ancestral-specific in a subject. The method comprises: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypcs and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; (h) annotating the subject's genome using the-ancestral-specific reference genome; and (i) identifying one or more genome sequence or exome sequence that are ancestral-specific in the subject.

In another aspect, the disclosure provides for a method for identifying one or more genome sequence or exome sequence that are ancestral-specific in a subject. The method comprises: (a) obtaining the subject's whole genome; (b) comparing the subject's whole genome to the subject's ancestral-specific reference database; (c) annotating the subject's genome based on the ancestral-specific reference database disclosed herein; and (d) identifying one or more genome sequence or exomc sequence'that are ancestral-specific in the subject.

In still another aspect, the disclosure provides a method of diagnosing a subject using, an ancestral specific reference genome. The method comprises: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite, familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that, are statistically significant; (g) preparing the ancestral-specific reference genome by'compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; (h) annotating the subject's genome using the ancestral-specific reference genome; and (i) identifying one or more genome sequence or exome sequence in the subject that differs from the one or more genome sequence or exome sequence in the ancestral-specific reference genome thereby identifying mutations or disease-causing variants in the one or more genome sequence or exome sequence of the subject's DNA to diagnose the subject.

In another aspect, the disclosure provides a method for diagnosing a subject using an ancestral specific reference genome. The method comprises: (a) obtaining the subject's whole genome; (b) comparing the subject's whole genome to the ancestral-specific reference genome; (c) annotating the subject's genome based on the ancestral-specific reference database disclosed herein; and (d) identifying one or more genome sequence or exome sequence in the subject that differs from the one or more genome sequence or exome sequence in the ancestral-specific reference genome thereby identifying mutations or disease-causing variants in the one or more genome sequence or exome sequence of the subject's DNA to diagnose the subject.

In another aspect the disclosure provides a method for diagnosing a subject at risk for a genetic disease, the method comprising: comparing a DNA sequence of the subject's whole genome with an ancestral-specific reference genome to identify mutations or disease-causing variants in the DNA sequence of the subject's whole genome DNA, wherein the ancestral-specific reference genome is prepared by compiling the statistically significant single nucleotide polymorphisms (SNPs) and/or haplotypes shared within groups of composite familial whole genome sequences, and the ancestral-specific reference genome compared with the DNA sequence of the subject's whole genome shares the statistically significant single nucleotide polymorphisms (SNPs) and/or haplotypes with the subject's composite familial whole genome data set. These SNPs and/or haplotypes are then used to identify mutations or disease-causing variants in the subject's genome to diagnose the subject.

In still another aspect the disclosure provides a method for diagnosing a subject using an ancestral specific reference genome obtained by: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide:polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; and (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference genome to diagnose the subject, wherein the subject is diagnosed with a genetic disease chosen from, but not limited to, Achromatopsia, Aicardi Syndrome, Albinism, Alexander Disease, Alpers' Disease, Alzheimer's Disease, Angelman Syndrome, Autism, Bardet-Biedl Syndrome, Barth Syndrome, Best's Disease, Bipolar Disorder, Bloom. Syndrome, Canavan Syndrome, Cancer, including Breast Cancer, Prostate Cancer, Ovarian Cancer, and other forms of cancer; including cancers resultant from germ-line and somatic mutations, Carnitine Deficiencies, Cerebral Palsy, Coffin Lowry Syndrome, Heart Defects, Hip Dysplasia, Cooley's Anemia, Corneal Dystrophy, Cystic Fibrosis, Cystinosis Diabetes, Down Syndrome, Epidermolysis Bullosa, Familial Dysautonomia, Fibrodysplasia, Fragile X Syndrome, Deficiency Anemia, Galactosemia, Gaucher Disease, Gilbert's Syndrome, Glaucoma, Hemochromatosis, Hemoglobin C Disease, Hemophilia/Bleeding Disorders, Hirschsprung's Disease, Homocystinuria, Huntington's Disease, Hurler Syndrome, Klinefelter Syndrome, Macular Degeneration, Marshall Syndrome, Menkes Disease, Metabolic Disorders, Microphthalmus, Mitochondrial Disease, Mucolipidoses, Muscular Dystrophy, Neonatal Onset. Multisystem Inflammatory Disease, Neural Tube Defects, Noonan Syndrome, Optic Atrophy, Osteogenesis Imperfecta, Peutz-Jeghers Syndrome, Phenylketonuria (PKU), Pseudoxanthoma Elasticum, Progeria, Scheie Syndrome, Schizophrenia, Sickle Cell Anemia, Skeletal Dysplasias, Spherocytosis, Spina Bifida, Spinocerebellar Ataxia, Stargardt Disease (Macular Degeneration), Stickler Syndrome, Toy-Sachs Disease, Thalassemia, Treacher Collins Syndrome, Tuberous Sclerosis, Turner's Syndrome, Urea Cycle Disorder, Usher's Syndrome, and Werner Syndrome.

In still another aspect, a method for determining a subject's ability to respond to an active agent is disclosed, where the steps of the method are determining an ancestral specific reference genome obtained by: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; and (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference genome to determine the ability to respond to the active agent. The active agent is preferably selected from a drug, biologic, or therapy.

In a further aspect, the present disclosure provides for a method of developing pharmaceuticals or therapy regimens having therapeutic efficacy in a sub-population, where the steps of the method are determining an ancestral specific reference genome obtained by: (a) obtaining a familial whole genome data set compfising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; and (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference genome to develop the pharmaceutical or therapy regimen for the sub-population.

In another aspect, the present disclosure provides for determining a sub-population of subjects for clinical trials, medical research, personalized medicine applications, and development of diagnostic technology, where, the steps of the method include determining an ancestral specific reference genome obtained by: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; and (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference genome to determine a sub-population of subjects for inclusion in clinical trials, medical research, personalized medicine applications, and development of diagnostic technology.

In an additional aspect, the present disclosure provides for using ancestral-specific genomes or exomes for determining personal attributes such as ancestry, gender, personal compatibility, a physical attribute, a biologic attribute, or genetic attribute where the steps of the method are determining an ancestral specific reference genome obtained by: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; and (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference genome to determine the personal attributes such as ancestry, gender, personal compatibility, a physical attribute, a biologic attribute, or genetic attribute.

Other features and embodiments are described in more detail herein.

5. BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 represents an exemplary sequence of steps to generate ancestral-specific reference genomes from large amounts of whole genome DNA sequence information.

FIG. 2 shows a diagrammatic representation of how statistically significant, ancestral-specific SNPs can be used to construct ancestral-specific reference genomes.

FIG. 3 represents an example of how ancestry specific reference genome information can be used to dectease the number of erroneous base calls generated by whole genome. DNA sequencing and the impact on the need to validate such erroneous base calls by orthogonal sequencing technology.

FIG. 4 shows a geographic distribution of countries of birth in a whole genome sequence database of the present disclosure. The ten large circles represent the ten ancestral genomes identified to date.

FIG. 5 shows the number of sequence variants by ancestry. Each column represents a different ethnicity. The number of variants is presented on the Y-axis. Individuals of African ancestry have the greatest number of variants when genomes are assembled to the standard NIH Reference Genome. By comparison, the North American and European Union genomes have the least variants, because they are derived from the same population used to generate the NIH Reference Genome.

FIG. 6 identifies individual genes that show ancestral-specific differences. The FLT3 gene only has variants in the African population. The PBX1 gene has variants in the American and Asian populations, but not the African and European populations. The TFRC gene only contains variants in the European population. All other genes have a similar number of variants in all ancestries. Population-based differences in variant number demonstrates the variability between populations at the genetic level and thus the importance of considering ancestry when sequencing a member of an ancestral population.

6. DETAILED DESCRIPTION OF THE INVENTION

Provided herein are ancestral-specific reference genome databases, methods for their construction, and uses, thereof. In certain embodiments, the ancestral-specific reference genome database comprises a plurality of ancestral-specific reference genomes that are statistically significant, familial corrected, and phased referenced. It is believed that, on a whole genome scal; there are thousands of differences between ancestral groups that significantly affect how individuals within these different ancestral groups react to drug therapies and that, when disease occurs, can impact their prognosis, diagnosis and therapy (landesbioscience.com/curie/chapter/3119/). Based on the observation that the DNA of individuals from different ancestral groups contains ancestral-specific differences that are important to the interpretation of genomic sequencing information (Kidd, J M; et al. (2008). “Mapping and sequencing of structural variation from eight human genomes”. Nature 453 (7191): 56-64. Bibcode 2008 Natur. 453 . . . 56K. doi:10.1038/nature06862. PMC 2424287. PM1D 18451855), a number of ancestral-specific and statistically significant reference genomes were generated using a sufficient number of sequenced genomes (currently greater than 10,000 whole genomes and growing). Such ancestral-specific genomes can be constructed for a whole or partial genome or exome. Further, an exomic genome, epigenic sequences, and ancestral-specific arrays can be prepared as described herein. Further, the present disclosure provides methods for determining a predisposition for a disease state, diagnosing a patient with a disease, preparing pharmaceuticals or treatment regimens for a sub-population, and more accurately interpret gcnomic sequencing information for medically relevant diagnostic and prognostic purposes.

Terminology

The following illustrative explanations are provided to facilitate understanding of certain terms used frequently herein, particularly in the examples. The explanations are provided as a convenience and are not limitative of the invention.

Partial Genome—any portion of the genome that is less than the whole genome, a single chromosome, region of the genome, introns, exons, intra-genic or inter-genic sequences, regulator elements, binding sites, enhancer sequences, and combinations thereof.

Diagnostic Marker—A gene or DNA sequence with a known location and sequence on a chromosome that can be used to identify individuals within a species, specifically used in the diagnosis of genetic disease.

Whole Genome Sequencing—A laboratory process that determines the complete DNA sequence of an organism's genome at a single time.

Medical-Grade DNA Sequencing—A laboratory process that determines the complete DNA sequence of an organism's genome at a single time utilizing techniques that conform to standard quality laboratory methods outlined by the Clinical Laboratory Administration Act (CLIA), identifying clinically relevant variants within a genome.

Haplotype—A group of alleles of different genes on a single chromosome that are linked closely enough to be inherited as a unit.

Ancestry—Persons initiating or comprising a line of descent.

Familial—Of or relating to a family, a group of people affiliated by consanguinity.

Database—A usually large collection of data organized especially for rapid search and retrieval (as by a computer).

Genome—One haploid or diploid set of chromosomes, depending on the organism, with the genes they contain; broadly: the genetic material of an organism.

Reference Genome—A digital nucleic acid sequence database, assembled by scientists as a representative example of a species' set of genes.

In silica—An expression used to mean “performed on computer or via computer simulation.”

Single Nucleotide Polymorphisms (SNPs)—A DNA sequence variation occurring when a single nucleotide in the genome differs between members of a biological species or paired chromosomes in a human.

Variant—A single nucleotide polymorphism or rare genetic substitution event depending on the frequency in the population.

Minor Allele—An alternative form of the same gene or same genetic locus found in the minority of the population.

Major Allele—An alternative form of the same gene or same genetic locus found in the majority of the population.

Exome—the part of the genome formed by exons, the sequences which when transcribed remain within the mature RNA after introns are removed by RNA splicing. An exotnic sequence reflects the expressed gene sequence.

Active agent—An active agent, for purposes of the present disclosure, refers to, but is not limited to, a pharmaceutical, drug, biologic, or therapy provided to a subject. A list of such pharmaceuticals, drugs, biologics, and therapy are provided herein.

Ancestral-specific—refers to genome or exome sequence changes that result from the ancestry of the subject, typically referring to attributes such as DNA sequence variants that are common to a group of individuals from the same geographic region or ethnicity and distinct from a group of individuals from a different geographic region or ethnicity.

6.1 Methods For Generating Ancestral-Specific Reference Genomes

The steps involved in generating the ancestral-specific reference genomes of such an ancestral-specific reference genome database are outlined in FIG. 1. The method described herein utilizes whole genome sequence data to generate familial whole genome data sets from multiple individuals within a family. This sequence is corrected for content by comparing the genome sequence of related individuals with the family to obtain a corrected familial whole genome data set. These corrected sequences are then compiled into a composite familial whole genome sequence, in such a manner that ancestral-specific differences between the genomes are identifiable. Familial genomes can then be added to a composite familial whole genome sequence until the composite familial whole genome sequence reaches statistical significance. Upon reaching statistical significance, the composite familial whole genome sequence is evaluated for the presence of SNPs and haplotype. Statistical probabilities are assigned to each SNP and haplotype and the composite familial whole genome sequence's are grouped according to statistically significant SNPs and/or haplotypes. The statistically significant SNPs and haplotypes can then be compiled into ancestral SNP and haplotype data sets. The ancestral-specific SNPs and/or haplotypes data sets can then be used to construct ancestral-specific reference genomes. A set of such ancestral-specific reference genomes describing ancestral and sub-ancestral groups can be utilized, for example, for medical diagnostics and research to target these groups, reducing the number of false positives and false negatives generated, and improving the efficiency of whole genome sequencing and enhancing performance of assays used in the development of personalized medicine applications.

The combination of familial-corrected sequences, ancestral-specific sequences, and statistical significance are all-critical to correcting the sequence to a sufficient level that the information can be used to evaluate a subject sample for mutations and disease-related SNPs. Without these corrections, the information obtained from DNA sequencing technologies generates so many false positives and false negatives that medical sequencing is currently outside of the realm of clinical utility as demonstrated in FIG. 3.

The geographic placement of the country of birth for individual genomes in ITMI's whole genome sequence database, currently comprising more than 10,000 whole genome sequences demonstrates that genomes are derived from 100 different countries. FIG. 4 shows how these countries of birth can be clustered into 10 ancestral, genomes. The size of the circle is proportional to the number of genomes from that country. As more genomes are added to the database, the number of countries will increase, however, the greatest increase will be in the statistical significance achieved by each reference genome.

The number of variants found in each genome is a function of the difference between that genome and the NIH reference genome that is currently used to assemble and align genomes during the sequencing process. The larger the number of variants found in a genome, the greater the need for a reference genome that accounts for ancestry. FIG. 5 shows genomes clustered by ancestry in columns as a function of the number of variants on the Y-axis. The African genomes differ the most from the NIH reference genome which is represented by the North American genomes. As genomes are assembled, variation from the NIH reference genome is represented by an increase in the number, of variants in a whole genome sequence. The consensus sequence from a group of genomes within an ancestry defines the basis of the reference genome that can be used for de novo assembly of genomes containing less variants and are thus more accurate representations of the individual genome.

At the genetic level ancestral variability is observed as differences in the number of variants in a gene. FIG. 6 shows the minor allele frequency for ten genes. Of the ten genes, there is ancestral variability within three. Using ancestral genomes would increase the ability to detect these differences at the genetic level and genomic level.

In one aspect, provided herein is a method for constructing an ancestral-specific reference genome database comprising the steps of: a) obtaining a familial whole genome data set, comprising whole genome DNA sequences from three or more individuals of a first family; b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; c) preparing a first composite'familial whole genome sequence from the corrected familial whole genome data set, wherein the first composite familial whole genome sequence comprises one or more single nucleotide polymorphisms (SNPs) and/or haplotypes; d) repeating steps a-c for second, third or more families to obtain second, third or more composite familial whole genome sequehces; e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and haplotypes and assigning statistical probabilities to each of the SNPs and haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; and g) preparing a plurality of ancestral-specific reference genomes, each ancestral-specific reference genome based on the statistically significant SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry.

In some embodiments, the method for constructing the ancestral-specific reference genome database comprises the step of obtaining a familial whole genome data set, comprising whole genome DNA sequences from three or more individuals of a first family. In certain embodiments, the whole genome and thus reference genome database includes the epigenome. Accordingly, the whole genome and thus reference genome database includes chemical changes to the DNA and histone proteins. Non-limiting examples of different aspects of the epigenome that may he included in the reference genome database are histone modifications; DNA methylation, acetylation and other chemical modification, chromatin accessibility, gene expression (e.g. mRNA) and small RNA expression (e.g. mRNA).

In certain embodiments, a method for constructing an ancestral-specific reference genome database comprises the steps of: a) obtaining a familial genome data set, comprising genome DNA sequences from three or more individuals of a first family; b) comparing the genome DNA sequences within the familial genome data set to obtain a corrected familial genome data set; c) preparing a first composite familial genome sequence from the corrected familial genome data set, wherein the first composite familial genome sequence comprises one or more single nucleotide polymorphisms (SNPs) and/or haplotypes; d) repeating steps a-c for second, third or more families to obtain second, third or more composite familial genome sequences; e) evaluating the first, second, third or more composite familial genome sequences for single nucleotide polymorphisms (SNPs) and haplotypes and assigning statistical probabilities to each of the SNPs and haplotypes; f) grouping the first, second, third or more composite familial genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; and g) preparing a plurality of ancestral-specific reference genomes, each ancestral-specific reference genome based on the statistically significant SNPs and/or haplotypes shared by a group of composite familial genome sequences with the same ancestry. Accordingly, a partial genome may be utilized to construct an ancestral-specific reference genome database. As used herein, a “partial gnome” may be a single gene, a single chromosome, a region of a chromosome or gene such as an intron, exon, intra-genic sequence, inter-genic sequence, regulatory clement, binding site, enhancer sequence. In all instances, the foregoing partial genome may contain more than one gene. chromosome, or region of a chromosome or gene. The partial genome may also contain the epigenome as described above. For example, the sequence of chromosome X or Y may be utilized to construct an ancestral-specific reference genome for those chromosomes.

In other embodiments, a method for constructing an ancestral-specific reference genome database comprises the steps of: a) obtaining a familial exome data set, comprising exome DNA sequences from three or more individuals of a first family; b) comparing the exome DNA sequences within the familial exome data set to obtain a corrected familial exome data set; c) preparing a first composite familial exome sequence from the corrected familial exome data set, wherein the first composite familial exome sequence comprises one or more single nucleotide polymorphisms (SNPs) and/or haplotypes; d) repeating steps a-c for second, third or more families to obtain second, third or more composite familial exome sequences; e) evaluating the first, second, third or more composite familial exome sequences for single nucleotide polymorphisms (SNPs) and haplotypes and assigning statistical probabilities to each of the SNPs and haplotypes; (f) grouping the first, second, third or more composite familial exome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; and g) preparing a plurality of ancestral-specific reference exomes, each ancestral-specific reference exome based on the statistically significant SNPs and/or haplotypes shared by a group of composite familial exome sequences with the same ancestry. Accordingly, a whole or partial exome may be utilized to construct an ancestral-specific reference database. When utilizing an exome database, only the expressed genes in the genome are sequenced. Accordingly, the exome database is limited to the coding regions of the genome. The whole or partial exome may also contain the epigenome as described above.

In an aspect of the present disclosure, the method further comprises an annotation step, where the sequence data are assembled and annotated for ancestral-specific significance. In certain embodiments, the ancestral-specific reference genome or database is used to annotate a subject's genome. Such an annotation step can be completed before the, process of creating the reference genome has begun, such as, but not limited to, during initial sequencing; at any point during the process of creating the reference genome; and/or after the reference genome has been created, as a filtering step. Further, such an annotation step can occur during initial sequencing and before the assembly of a subject's whole or partial genome or whole or partial exome. Accordingly, the method provided herein reduces the number of unknown variants in a subject's genome. For example, prior art methods result in thousands or millions of unknown variants whereas the disclosed method can decrease the number of unknown variants to a greatly reduced number (10 s or 100 s of variants less). Thus, by using an ancestral-specific reference genome, a disease-causing mutation can be distinguished from an ancestral-specific mutation.

As used herein, the term “subject” or “individuals” is used to refer to a human; an animal, including, but not limited to, a livestock animal, a companion animal, a lab animal, a zoological animal, a bird, and a mammal; an insect; a reptile; an organism or microorganism; and a plant. In one aspect, the individual may be a rodent, e.g. a mouse, a rat, a guinea pig, etc. In another aspect, the individual may be a livestock animal. Non-limiting examples of suitable livestock animals may include pigs, cows, horses, goats, sheep, llamas and alpacas. In yet another aspect, the individual may be a companion animal. Non-limiting examples of companion animals may include pets such as dogs, cats, rabbits, and birds. In yet another aspect, the individual may be a zoological animal. As used herein, a “zoological animal” refers to an animal that may be found in a zoo. Such animals may include non-human primates, large cats, wolves, and bears. In certain aspects, the animal is a laboratory animal. Non-limiting examples of a laboratory animal may include rodents, canines, felines, and non-human primates. In a specific aspect, the individual is human.

As used herein, the term “family” refers to a group of individuals, related by genetic material, including individuals related to each other by the first degree (e.g., parents, full siblings, and children), second degree (grandparents, grandchildren, aunts, uncles, nephews, nieces and half-siblings), or third degree (first-cousins, great grandparents, and great grandchildren). In some embodiments, the familial whole genome data set comprises whole genome DNA sequences from four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, nineteen, twenty or more individuals of a family. The “family” also applies to organisms and plants and can be determined based on genetic lineage. In some embodiments, the familial whole genome data set comprises whole genome DNA sequences from individuals of a family related to each other by ten degrees or less, by nine degrees or less, by eight degrees or less, by seven degrees, by six degrees or less, by five degrees, by four degrees or less, by three degrees or less, by two degrees or less, or by one degree.

Obtaining a familial whole genome data set, comprising whole genome DNA sequences from multiple individuals can be performed by any method known to those skilled in the art. In certain embodiments, the whole genome DNA sequences are obtained by performing a DNA sequencing reaction on whole genome DNA from three or more individuals from the same family. A DNA sequencing reaction can be performed using a commercially available sequencer such as those developed by Sanger (Sanger F, Coulson A R (May 1975). “A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase”. J. Mol. Bid. 94 (3): 441-8. doi:10.1016/0022-2836(75)90213-2. PMID 1100841), Life Technologies (invitrogen.com/site/us/en/home/Products-and-Services/Applications/Sequencing/Semiconductor-Sequencing/proton.html), Pacific Biosciences (pacificbiosciences.com/), Illumina (illumina.com/) and Complete Genomics (completegenomics.com/) for example. In other embodiments, the whole genome DNA sequences are obtained from publicly available databases, including, but not limited to, databases developed by the International HapMap Project (hapmap.ncbi.nlm.nih.gov/); the National Center for Biotechnology Information, National Institutes of Health, Bethesda, Md. (NCBI) (ncbi.nlm.nih.gov/); and the European Molecular Biology Laboratory (EMBL) Nucleotide Sequence Database, Heidelberg, Germany (ebi.ac.uk/embl). In specific embodiments, the whole genome DNA sequences may, in part, be obtained from HapMap populations from the International HapMap Project.

In some embodiments, the method for constructing the ancestral-specific reference genome database comprises the step of comparing each whole genome DNA sequence within a familial whole genome data set, in whole or in part, against one another to obtain a corrected familial whole genome data set. In specific embodiments, the step of comparing whole genome. DNA sequences within a familial whole genome data set comprises comparing every base position of a whole genome DNA sequence against other whole genome DNA sequences within the familial whole genome data set to determine differences in DNA sequences among the whole genome DNA sequences of the familial whole genome data set. In particular embodiments, a difference observed at a base position among the DNA sequences in a familial whole genome data set is validated using an orthogonal technology (e.g., two or more genoine sequencing methods as described infra) to determine if the difference is due to an artifact of the platform used (e.g., an erroneous base call on the platform) or if the difference is a true nucleotide change. Differences in sequences due to errors are corrected to produce a corrected familial whole genome data set.

In certain embodiments, the method comprises the step of assembling the whole or partial genome or exome DNA sequence of a subject's DNA by annotating the whole or partial genome or exome DNA sequence of the subject's DNA using the ancestral-specific reference genome to determine the ancestral-specific genes or exons present within the genome or exome. This step allows for a disease-specific mutation to be distinguished from an ancestral-specific mutation. Accordingly, this step allows for less unknown variants and provides a more accurate diagnosis with reduced false positives and false negatives. Specifically, ancestry-specific sequences are identified in blocks of nucleotides. For example, the blocks of nucleotides can be 1000 nucleotides or fewer, 100 nucleotides or fewer, or 10 nucleotides or fewer. The ancestry of the subject is determined by comparing the block of nucleotides to the ancestral-specific reference database to determine the appropriate ancestral-specific reference genome for the subject. Accordingly, sequences in these blocks are ancestral in nature and the ancestral-specific reference genome identified represents an appropriate background for the identification of a polymorphism or disease-specific mutation. Thus, the ancestral blocks of DNA sequence can be annotated on the whole genome making it possible to distinguish between polymorphisms that occur randomly (i.e. disease specific) from polymorphisms that occur within an ancestral group of individuals. In certain embodiments, this annotation step occurs during the process of producing the ancestral-specific reference genome. In other embodiments, the annotation step is used as a filtering step after the ancestral-specific reference genome is produced. In an additional embodiment, the annotation step occurs both during the process of producing the ancestral-specific reference genome and as a filtering steps after the ancestral-specific reference genome is produced.

In some embodiments, the method for constructing the ancestral-specific reference genome database comprises the step of preparing a composite familial whole genome sequence, in whole or in part, from the corrected familial whole genome data set, wherein the composite familial whole genome sequence comprises one or more single nucleotide polymorphisms (SNPs) and/or haplotypes. Such composite familial whole genome sequences can be constructed, for example, using the information provided by the corrected familial whole genome data set, familial inheritance patterns and specifically developed analytic tools and algorithms.

In particular embodiments of the method, the steps of a) obtaining a familial whole genome data set, comprising whole genome DNA sequences; in whole or in part, from three or more individuals of a family; b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; c) preparing a composite familial whole genome sequence from the corrected familial whole genome data set, are repeated for a second, third or more families to obtain a second, third or more composite familial whole genome sequences. In certain embodiments of the method, the steps are repeated for 2 or more, 3 or more, 4 or more, 5 or more, 6 or more, 7 or more, 8 or more, 9 or more, 10 or more, 11 or more, 12 or more, 13 or more, 14 or more, 15 or more, 16 or more, 17 or more, 18 or more, 19 or more, 20 or more, 30 or more, 40 or more, 50 or more, 60 or more, 70 or more, 80 or more, 90 or more, or 100 or more families to obtain composite familial whole genome sequences for each of the families.

In particular embodiments, the method described herein comprises the step of evaluating the composite familial whole genome sequences, in whole or in part, for single nucleotide polymorphisms and/or haplotypes and assigning statistical probabilities to each of the SNPs and/or haplotypes. Any method known to those skilled in the art can be used to evaluate the presence of single nucleotide polymorphisms and haplotypes, including analytical tools that are available in the public domain (see, e.g., HaploView, broadinstitute.org/scientific-community/science/programs/medical-and-population-genctics/haploview/haploview). Statistical significance of the SNPs and haplotypes are then determined for each SNPs and haplotype that are evaluated. A SNP is an “ancestral-specific SNP” if a particular allele of the SNP occurs at a frequency of greater than 99%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55% or 50% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiments, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 99% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiments, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 95% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiments, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 90% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiments, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 85% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiments, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 80% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiments, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 75% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiments, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 70% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiinents, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 65% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In certain embodiments, a SNP is an “ancestral-specific SNP” if it occurs at a frequency of greater than 60% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. A haplotype is “ancestral-specific” if a particular haplotype occurs at a frequency of greater than 99%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55% or 50% as compared to the frequency at which it occurs in another distinct composite familial whole genome sequence. In particular embodiments, these ancestral-specific SNPs/haplotypes are then used to generate ancestral-specific reference genomes of the ancestral-specific reference genome database.

In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 1×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 1.5×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 2.0×10⁶ or more SNPs.

In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 2.5×10⁶ or more SNPs.

In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 3×10⁶ or more SNPs.

In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 3.5×10⁶ or more SNPs.

In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 4×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 4.5×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 5×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 5.5×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 6×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 6.5×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 7×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 7.5×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 8×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 8.5×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 9×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 9.5×10⁶ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 1×10⁷ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 1.5×10⁷ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 2×10⁷ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral/specific reference genome database comprises up to and including 3×10⁷ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 4×10⁷ or more SNPs. In certain embodiments, an ancestral-specific reference genome of the ancestral-specific reference genome database comprises up to and including 5×10⁷ or more SNPs. The ancestral-specific SNPs identified using this method can be used to generate a composite ancestral-specific reference genome for each ancestral group analyzed.

The method described above can then be repeated and refined using subsets of individuals from each ancestral group. For example, the European ancestral-specific reference genome may be subdivided into an Eastern European-specific reference genome, Northern European specific reference genome, etc.

The triangle shown in FIG. 2 depicts how this information is used to generate ancestral-specific reference genomes. Each of the corners of the triangle shown in FIG. 2 represents an ancestral group, i.e., European, African, or Asian. Markers that plot at the corners of the triangle represent ancestral-specific SNPs. For example, points that plot in the corner at the bottom right-hand sector of the triangle represent SNPs that are specific to individuals of European ancestry, because these variants occur in individuals of European ancestry, but not in individuals of African or Asian ancestries.

6.2 Uses of Ancestral-Specific Reference Genomes

The ancestral-specific reference genomes in whole or in part described herein have applications in the fields of analysis, DNA-based diagnostics, DNA sequencing, pharmaceutical drug development and clinical application of genomic information. These reference genomes make it possible to analyze whole genome or exome sequence data to generate more meaningful results by eliminating false positives and false negatives from the sequence data. The improved accuracy provided by ancestral-specific reference genomes permit the elimination of erroneous data. See FIG. 3.

The more accurate set of SNP and/or haplotype data generated from the results of this analysis may be placed in the context of other data, such as proteomic or pathway data, resulting in a more accurate interpretation of the impact of SNPs and/or haplotypes in the context of disease or for other applications as described in the examples listed below.

In an aspect, if a disease-specific SNP is identified, the individual may be diagnosed with or have a predisposition for said disease. For example, if a disease-specific SNP is identified, the individual may be at least 1.2-fold, at least 1.3-fold, at least 1.4-fold, at least 1.5-fold, at least 2-fold, at least 3-fold, at least 4-fold, at least 5-fold, at least 10-fold, at least 15-fold, at least 20-fold, at least 25-fold, at least 30-fold, at least 35-fold, at least 40-fold, at least 45-fold, at least 50-fold, at least 75-fold, or at least 100-fold more likely to be diagnosed with said disease. Alternatively, if a disease-specific SNP is identified, the individual may be at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, or at least 95% more likely to be diagnosed with said disease. Further, if a disease-specific SNP is identified, the individual may be significantly more likely to be diagnosed with said disease. If an individual is significantly more likely, the p-value is less than 0.1, preferably less than 0.05, more preferably less than 0.01, even more preferably less than 0.005, the most preferably less than 0.001 relative to an individual that does not possess the disease-specific SNP.

In another aspect, the constructed ancestral-specific reference genome may be used to create one or more sequence arrays. Accordingly, a disease-specific variation identified in a specific ancestral group may be used to generate an array. In such a manner, a genome from an individual may be contacted with the specific ancestral group array such that a disease-specific variation is identified. Thus, the array is specific to the individual's ancestral group and can reliably identify a disease-specific variation. An array may be developed for a partial genome, a whole genome, a partial exome, or a whole exome. The array may contain epigenome features. In certain embodiments, the constructed ancestral-specific reference genome may be used for annotation. The ancestral-specific reference genome can be used to generate a database of ancestral information. Accordingly, various sequences that define an ancestry may be identified.

7. EXAMPLES

7.1 Enhanced Diagnostics

The field of DNA-based diagnostics relies on the ability to accurately identify DNA sequence, specifically in the nucleotide residues that result in disease-causing sequence variation. Accuracy of variant identification by sequence analysis is a major rate limiting step in the development of novel diagnostic markers and their use in testing the population. Variants identified utilizing enhanced reference genome translates into more accurate diagnostic markers and more accurate diagnostic tests. The utility of the reference genome for improving variant identification is independent of the technology used to generate variant information. By applying the information contained in the reference genome to the sequence technology utilized to generate the variant information, the interpretation of the variant information in enhanced. These markers can be used for prognostic or diagnostic testing for counseling of patients or as companion diagnostics for pharmaceutical compounds.

There are almost 1000 gene/SNP specific diagnostic tests available for medical diagnostics. This number is relatively small compared to the large number of potential disease-causing variants in the genome. The disease-causing variants that occur in genetic disorders which may be identified or determined for purposes of the present disclosure include, but are not limited to: Achromatopsia, Aicardi Syndrome, Albinism, Alexander Disease, Alpers' Disease, Alzheimer's Disease, Angelman Syndrome, Autism, Bardet-Biedl Syndrome, Barth Syndrome, Best's Disease, Bipolar Disorder, Bloom Syndrome, Canavan Syndrome, Cancer, including Breast Cancer, Prostate Cancer, Ovarian Cancer, and other forms of cancer, including cancers resultant from germ-line and somatic mutations, Carnitine Deficiencies, Cerebral Palsy, Coffin Lowry Syndrome, Heart Defects, Hip Dysplasia, Cooley's Anemia, Corneal Dystrophy, Cystic Fibrosis, Cystinosis Diabetes, Down Syndrome, Epidermolysis Bullosa, Familial Dysautonomia, Fibrodysplasia, Fragile X Syndrome, Deficiency Anemia, Galactosemia, Gaucher Disease, Gilbert's Syndrome, Glaucoma, Hemochromatosis, Hemoglobin C Disease, Hemophilia/Bleeding Disorders, Hirschsprung's Disease, Homocystinuria, Huntington's Disease, Hurler Syndrome, Klinefelter Syndrome, Macular Degeneration, Marshall Syndrome, Menkes Disease, Metabolic Disorders, Microphthalmus, Mitochondrial Disease, Mucolipidoses, Muscular Dystrophy, Neonatal Onset Multisystem Inflammatory Disease, Neural Tube Defects, Noonan Syndrome, Optic Atrophy, Osteogenesis Imperfecta, Peutz-Jeghers Syndrome, Phenylketonuria (PKU), Pseudoxanthoma Elasticum, Progeria, Scheie Syndrome, Schizophrenia, Sickle Cell Anemia, Skeletal Dysplasias, Spherocytosis, Spina Bifida, Spinocerebellar Ataxia, Stargardt Disease (Macular Degeneration), Stickler Syndrome, Toy-Sachs Disease, Thalassemia, Treacher Collins Syndrome, Tuberous Sclerosis, Turner's Syndrome, Urea Cycle Disorder, Usher's Syndrome or Werner Syndrome, Glycogen Branching Enzyme Deficiency (GBED), Hereditary Equine Regional Dermal Asthenia (HERDA), Hyperkalemic Periodic Paralysis Disease (HYPP), Malignant Hyperthermia (MH), Polysaccharide Storage Myopathy—Type 1 (PSSM1), a-Mannosidosis, blood group incompatibility, neonatal isoerythrolysis, Burmese Head Defect, Deafness, Devon Rex Myopathy, Gangliosidosis, Glycogen storage disease type IV, Hypertrophic Cardiomyopathy, Hypertrophic Muscular dystrophy, Hypokalameic Polymyopathy, Manx Syndrome (spina bifida), Mucopolysaccharidosis, Niemann-Pick Disease, Osteochrondrodysplasia or Scottish Fold disease, Polycystic kidney disease, Polydactyl cats, Progressive retinal atrophy, Pyruvate kinase deficiency, Spinal muscular atrophy, Osteoarthritis, Hip dysplasia, Elbow dysplasia, Luxating patella, Osteochondritis dissecans (OCD), panosteitis, Legg-Calvé-Perthes syndrome, Congenital vertebral anomalies, Craniomandibular osteopathy, Spondylosis, Masticatory muscle myosis, von Wilebrand disease, Thrombocytopenia, Thromboystosis, Hemolytic anemia, Tetra-logy of Fallot, Patent ductus arteriosis, Heart valve dysplasia, Cor triatriatum, Subvalvular Aortic stenosis, Pulmonic stenosis, Ventricular septal defect, Atrial septal defect, Epilepsy, Eyelid diseases, Retinal disease, Lens diseases, Corneal diseases, and Endocrine Diseases.

7.2 Ancestral-Specific Pharmaceutical Development

The development of pharmaceutical compounds is currently limited by the ability to identify groups within the general population that respond either favorably or unfavorably to a pharmaceutical compound. For example, it is possible to develop a pharmaceutical compound that has therapeutic efficacy in a sub-population, but the therapeutic effect may be obscured because that sub-population represents a minority in the general population. Similarly, it is possible to develop a pharmaceutical compound that has therapeutic efficacy in one sub-population, but has significant deleterious side effects in another sub-population. For this reason, it is advantageous to develop and evaluate pharmaceutical compounds at the sub-population level. The ancestral-specific nature of these reference genomes is critical to the development of ancestral-specific pharmaceutical compounds. As pharmaceutical companies are encouraged by the Food and Drug Administration (FDA) and economic factors to produce more narrowly focused therapeutics and diagnostics, these reference genomes provide the ability to determine in advance if a therapeutic is effective in a subgroup of the population.

As used herein, an “active agent” preferably includes, but is not limited to one or more pharmaceutical compounds, a chemotherapeutic agents, anti-metabolites, anti-tumor antibiotics, anti-cytoskeletal agents, topoisomerase inhibitors, targeted therapeutic agents, angiogenesis inhibitors, growth inhibitory polypeptides, antincoplastic agents, anti-viral agents, drugs, biologics, therapy regimens, and combinations thereof. A “pharmaceutical compound,” for purposes of the present disclosure is any compound known in the art that is used in the detection, diagnosis, or treatment of a condition or disease. Such compounds may be naturally-occurring, modified, or synthetic. Non-limiting examples of pharmaceutical compounds may include drugs, therapeutic compounds, genetic materials, metals (such as radioactive isotopes), proteins, peptides, carbohydrates, lipids, steroids, nucleic acid based materials, pesticides, or derivatives, analogues, or combinations thereof in their native form or derivatized with hydrophobic or charged moieties to enhance incorporation or adsorption into a cell. Such pharmaceutical compounds may be water soluble or may be hydrophobic. Non-limiting examples of pharmaceutical compounds may include immune-related agents, thyroid agents, respiratory products, antineoplastic agents, anti-helmintics, anti-malarials, mitotic inhibitors, hormones, anti-protozoans, anti-tuberculars, cardiovascular products, blood products, biological response modifiers, anti-fungal agents, vitamins, peptides, anti-allergic agents, anti-coagulation agents, circulatory drugs, metabolic potentiators, anti-virals, anti-anginals, antibiotics, anti-inflammatories, anti-rheumatics, narcotics, cardiac glycosides, neuromuscular blockers, sedatives, local anesthetics, general anesthetics, or radioactive atoms or ions. Non-limiting examples pharmaceutical compounds are described below. In certain aspects, a pharmaceutical compound may be a compound used in the treatment of cancer.

A pharmaceutical compound may be a small molecule therapeutic, a therapeutic antibody, a therapeutic nucleic acid, or a chemotherapeutic agent. Non-limiting examples of therapeutic antibodies may include muromomab, abciximab, rituximab, daclizumab, basiliximab, palivizumab, infliximab, trastuzumab, etanercept, gemtuzumab, alemtuzumab, ibritomomab, adalimumab, alefacept, omalizumab, tositumomab, efalizumab, cetuximab, bevacizumab, natalizumab, ranibizumab, panitumumab, eculizumab, and certolizumab. A representative therapeutic nucleic acid may encode a polypeptide having an ability to induce an immune response and/or an anti-angiogenic response in vivo. Representative therapeutic proteins with immunostimulatory effects include but are not limited to cytokines (e.g., an interleukin (IL) such as IL2, IL4, IL7, IL12, interferons, granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor alpha (TNF-α)), immunomodulatory cell surface proteins (e.g., human leukocyte antigen (HLA proteins), co-stimulatory molecules, and tumor-associated antigens. See Kirk & Mule, 2000; Mackensen et al., 1997; Walther & Stein, 1999; and references cited therein. Representative proteins with anti-angiogenic activities that can be used in accordance with the presently disclosed subject matter include: thrombospondin I (Kosfeld & Frazier, 1993; Tolsma et al., 1993; Dameron et al., 1994), metallospondin proteins (Carpizo & Iruela-Arispe, 2000), class I interferons (Albini et al., 2000), IL12 (Voest et al., 1995), protamine (Ingber et al., 1990), angiostatin (O'Reilly et al., 1994), laminin (Sakamoto et al., 1991), endostatin (O'Reilly et al., 1997), and a prolactin fragment (Clapp et al., 1993). In addition, several anti-angiogenic peptides have been isolated from these proteins (Maione et al., 1990; Eijan et al., 1991; Woltering et al., 1991). Representative proteins with both immunostimulatory and anti-angiogenic activities may include IL12, interferon-γ, or a chemokine. Other therapeutic nucleic acids that may be useful for cancer therapy include but are not limited to nucleic acid sequences encoding tumor suppressor gene products/antigens, antimetabolites, suicide gene products, and combinations thereof.

A chemotherapeutic agent refers to a chemical compound that is useful in the treatment of cancer. The compound may be a cytotoxic agent that affects rapidly dividing cells in general, or it may be a targeted therapeutic agent that affects the deregulated proteins of cancer cells. A cytotoxic agent is any naturally-occurring, modified, or synthetic compound that is toxic to tumor cells. Such agents are useful in the treatment of neoplasms, and in the treatment of other symptoms or diseases characterized by cell proliferation or a hyperactive cell population. The chemotherapeutic agent may be an alkylating agent, an anti-metabolite, an anti-tumor antibiotic, an anti-cytoskeletal agent, a topoisomerase inhibitor, an anti-hormonal agent, a targeted therapeutic agent, a photodynamic therapeutic agent, or a combination thereof.

Non-limiting examples of suitable alkylating agents may include altretamine, benzodopa, busulfan, carboplatin, carboquone, carmustine (BCNU), chlorambucil, chlornaphazine, cholophosphamide, chlorozotocin, cisplatin, cyclosphosphamide, dacarbazine (DTIC), estramustine, fotemustine, ifosfamide, improsulfan, lipoplatin, lomustine (CCNU), mafosfamide, mannosulfan, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, meturedopa, mustine (mechlorethamine), mitobronitol, nimustine, novembichin, oxaliplatin, phenesterine, piposulfan, prednimustine, ranimustine, satraplatin, semustine, temozolomide, thiotepa, treosulfan, triaziquone, triethylenemelamine, triethylenephosphoramide (TEPA), triethylenethiophosphaoramide (thiotepa), trimethylolomelamine, trofosfamide, uracil mustard and uredopa.

Suitable anti-metabolites may include, but are not limited to aminopterin, ancitabine, azacitidine, 8-azaguanine, 6-azauridine, capecitabine, carmofur (1-hexylcarbomoyl-5-fluorouracil), cladribine, clofarabine, cytarabine (cytosine arabinoside (Ara-C)), decitabine, denopterin, dideoxyuridine, doxifluridine, enocitabine, floxuridine, fludarabine, 5-fluorouracil, gemcitabine, hydroxyurea (hydroxycarbamide), leucovorin (folinic acid), 6-mercaptopurine, methotrexate, nafoxidine, nelarabine, oblimersen, pemetrexcd, pteropterin, raltitrexed, tegofur, tiazofurin, thiamiprine, tioguanine (thioguanine), and trimetrexate.

Non-limiting examples of suitable anti-tumor antibiotics may include aclacinomysin, aclarubicin; actinomycins, adriamycin, auristatin (for example, monomethyl auristatin E), authramycin, azaserine, bleomychis, cactinomycin, calicheamicin, carabicin, caminomycin, carzinophilin, chromomycins, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin, epirubicin, epoxomicin, esorubicin, idarubicin, marcellomycin, mitomycins, mithramycin, mycophenolic acid, nogalamycin, olivomycins, peplomycin, plicamycin, potfiromycin, puromycin, quelamycin, rodorubicin, sparsomycin, streptonigrin, streptozocin, tubercidin, valrubicin, ubenimex, zinostatin, and zorubicin.

Non-limiting examples of suitable anti-cytoskeletal agents may include cabazitaxel, colchicines, demecolcine, docetaxel, epothiloncs, ixabepilone, macromycin, omacetaxine mepesuccinate, ortataxel, paclitaxel (for example, DHA-paclitaxel), taxane, tesetaxel, vinblastine, vincristine, vindesine, and vinorelbine.

Suitable topoisomerase inhibitors may include, but are not limited to, amsacrine, etoposide (VP-16), irinotecan, mitoxantrone, RFS 2000, teniposide, and topotecan.

Non-limiting examples of suitable anti-hormonal agents may include aminoglutethimide, antiestrogen, aromatase inhibiting 4(5)-imidazoles, bicalutamide, finasteride, flutamide, fluvestrant, goserelin, 4-hydroxytamoxifen, keoxifene, leuprolide, LY117018, mitotane, nilutamide, onapristone, raloxifene, tamoxifen, toremifene, and trilostane.

Examples of targeted therapeutic agents may include, without limit, monoclonal antibodies such as alemtuzumab, cartumaxomab; edrecolomab, epratuzimab, gemtuzumab, gemtuzumab ozogamicin, glembatumumab vedotin, ibritumomab tiuxetan, reditux, rituximab, tositumomab, and trastuzumab; protein kinase inhibitors such as bevacizumab, cetuximab, crizonib, dasatinib, erlotinib, gefitinib, imatinib, lapatinib, mubritinib, nilotinib, panitumumab, pazopanib, sorafenib, sunitinib, toceranib; and vandetanib.

Non limiting examples of angiogeneisis inhibitors may include angiostatin, bevacizumab, denileukin diftitox, endostatin, everolimus, genistein, interferon alpha, interleukin-2, interleukin-12, pazopanib, pegaptanib, ranibizumab, rapamycin (sirolimus), temsirolimus, and thalidomide.

Non limiting examples of growth inhibitory polypeptides may include bortazomib, erythropoietin, interleukins (e.g., IL-1, IL-2, IL-3, IL-6), leukemia inhibitory factor, interferons, romidepsin, thrombopoietin, TNF-α, CD30 ligand, 4-1BB ligand, and Apo-1 ligand.

Non-limiting examples of photodynamic therapeutic agents may include aminolevulinic acid, methyl aminolevulinate, retinoids (alitretinon, tamibarotene, tretinoin), and temoporfin.

Other antineoplastic agents may include anagrelide, arsenic trioxide, asparaginase, bexarotene, bropirimine, celecoxib, chemically linked Fab, efaproxiral, etoglucid, ferruginol, lonidamide, masoprocol, miltefosine, mitoguazone, talapanel, trabectedin, and vorinostat.

Also included are pharmaceutically acceptable salts, acids, or derivatives of any of the above listed agents.

Other pharmaceutical compounds may comprise a virus, or a viral genome such as an oncolytic virus. An oncolytic virus comprises a naturally occurring virus that is capable of killing a cell in the target tissue (for example, by lysis) when it enters such a cell.

7.2 Medical-Grade DNA Sequencing

Current DNA sequencing using the existing reference genomes is for research purposes only. Companies that claim to perform medical-grade DNA sequencing are utilizing research quality materials and methods in a CLIA environment to evaluate a limited number of variants in a small subset of the genes contained within the genome. The false positive and false negative errors introduced into the DNA sequence are the combined result of technological issues and the use of an inaccurate reference genome. Use of the ancestral reference genomes described herein provides a more accurate DNA sequencing method for the development of medical sequencing on a commercially feasible scale.

Currently, all DNA sequencing companies utilize the existing NIH reference genome; however, tailoring the reference to the particular genealogic background of the individual improves efficiency and accuracy of the final product. The current NIH reference genome is of limited utility because the sequence was generated from the DNA of only five individuals without regard to ancestry. Numerous versions of the NIH reference genome have been generated, correcting the reference sequence utilizing a variety of different datasets that also contain no ancestral information. The result is a reference genome that lacks statistical significance and haplotype information, and focuses only on major variants found in a single ancestry. Often, only minor variants are identified for nucleotide positions within the genome, or no call can be made based on the inability for current base-calling software to distinguish between two or more variants localized to the same nucleotide position. Ancestral-specific reference genomes that have been corrected with familial and haplotype information provide a mechanism for improving the quality of DNA sequencing to the point where it is medically useful.

The use of the ancestral reference whole genomes, partial genomes, whole exomes, and partial exomes enhances the ability of clinicians to apply genomic information to their patients. If the genealogy of a patient is known or can be determined by the DNA sequence of the individual or family members, the clinician can use that information to determine which therapy may best suit the needs and the safety of the patient based on the availability of ancestral-specific therapeutic compounds.

7.3 Identification of Personal Attributes for Non-Medical Purposes

In another aspect, provided herein is an example of using ancestral-specific reference genomes, in whole or in part; for non-medical applications which utilize whole genomic sequence, partial genomic sequence, whole exome sequence, partial exome sequence, and SNP data to inform an individual about personal attributes such as ancestry, gender, compatibility between individuals based on actual or perceived physical, biological or psychological attributes, genetic compatibility or other information that can be obtained about an individual from their sequence information. This example specifically enables individuals to learn more about potential partners by comparing genomic information that has been enhanced for accuracy with ancestral-specific reference information. Other applications also exist. For example, individuals may use the reference genomes to compare the variant profile of their genes for physical ability, intellectual capacity or musical talent with a reference genome to improve the accuracy of comparisons.

In one embodiment, the method for identifying an individual attribute in an individual such as ancestry, personal compatibility, a physical attribute, a biological attribute, a psychological attribute or genetic compatibility, comprises the step of comparing a DNA sequence of an individual with any one or more of the ancestral-specific reference genomes of the ancestral-specific reference genome databases, wherein the one or more ancestral-specific reference genomes comprises one or more single nucleotide polymorphisms and/or haplotypes associated with a known individual attribute, and determining whether the DNA sequence of the individual also comprises the one or more single nucleotide polymorphisms and/or haplotypes associated with the known individual attribute.

7.4 Forensic Science Applications

In certain embodiments, the methods of using the ancestral-specific reference genome databases for forensic applications include, but are not limited to, paternity testing, improving identification of living or deceased individuals where conventional methods of identification fail, such as in a bomb blast, mass grave or natural disasters such as earthquakes and tidal waves. In the event that conventional methods of identification, such as fingerprint analysis, dental record review or DNA specific information that can be used to identify a person, comparison to reference genomes can provide information about a person's ancestry. For example, such an analysis could determine if a deceased individual is of Northern European versus Southern European descent, providing rescue groups or law enforcement or government agencies with information about a person's identity that they otherwise would not have.

7.5 Law Enforcement Applications

In other embodiments, the ancestral-specific reference genome databases and methods provided herein may be used in law-enforcement applications, such as the ancestral classification of an individual when a sample of their DNA is available that does not match an individual in law enforcement databases. Under such conditions, an unknown individual's DNA is used to determine the ancestry of the individual, making it possible to eliminate individuals outside of that ancestry as suspects and focusing the search for the guilty party to individuals from a specific ancestry. In another embodiment, ancestral reference genomes is used by government agencies such as the FBI or Department of Homeland Security to identify the ancestry of persons of interest such as terrorists, thus narrowing the search for persons of interest to a specific ancestry. In another embodiment, ancestral-specific reference genomes are applied to DNA-based information contained within FBI databases to improve the accuracy of identification of an individual. The improved accuracy resulting from the use of ancestral-specific reference genomes increases the statistical likelihood that the FBI has arrested the correct individual.

7.6 Reproduction Technologies

In another aspect, a method of using one or more ancestral-specific reference genome(s), in whole or in part, of an ancestral-specific reference genome database described herein for the selection of embryos, eggs or sperm for artificial reproduction. This includes the genetic evaluation of embryos, eggs and sperm for the detection of genetic disease, genomic disease, pharmacogenomic applications, and determination of relatedness of individuals or the selection of physical attributes such as eye color or hair color or the identification of other attributes of interest, to couples, physicians, or scientists.

This also relates to paternity testing and to the typing of embryos for in vitro fertilization to minimize ancestral-related diseases such as in founder situations in inbred populations such as the Amish and Ashkenazi Jewish populations and to minimize the risk of genetic disease from reproduction by related individuals. In some embodiments, the method comprises the step of comparing a DNA sequence of an embryo, egg and/or sperm with any one or more of the ancestral-specific reference genomes of the ancestral-specific reference genome database of claim 1, wherein the one or more of the ancestral-specific reference genomes comprises one or more single nucleotide polymorphisms and/or haplotypes associated with a known genetic diseases, genomic attribute or physical characteristic, and determining whether the DNA sequence of the individual also comprises the one or more single nucleotide polymorphisms and/or haplotypes associated with the known genetic diseases, genomic attribute or physical characteristic. In some embodiments, the method comprises the step of comparing a DNA sequence of a sperm or egg of a first individual and the DNA sequence of a sperm or egg of a second individual with one or more ancestral-specific reference genomes, in whole or in part, of an ancestral-specific reference genome database described herein to determine the relatedness of the first individual and the second individual. The use of ancestral-specific reference genomes makes the analysis more accurate that current sequence analysis that utilizes the existing reference genome and thus increases the likelihood of the preferred outcome.

7.7 Non-Human Uses

In another aspect, provided herein is a method of using ancestral reference genomes, in whole or in part in other species for the selection of attributes. This includes, but is not limited to, the use of human and non-human reference genomes for identification of recombinant organisms that contain desired genotypes that may or may not confer a phenotype in the individual or lineage being evaluated. In one example, a “humanized mouse” animal model created in the laboratory to contain a part of or an entire human chromosome is evaluated for functional genes or DNA sequences contained in the hybrid. The advantage of utilizing ancestral-specific reference genomes is the improve accuracy of the DNA sequencing performed on these samples to ensure that the researcher is utilizing organisms that carry the variants necessary to achieve research goals.

In another embodiment, the reference genomes is used to improve the accuracy with which, eggs, sperm or embryos are identified for the selective breeding of livestock, or the selection of microorganisms for research or industrial purposes, similar to its use in humans for reproductive technologies. In such instances, an organism-specific reference genome is created to facilitate the discrimination between different variants.

In still another embodiment, the reference genome is used to improve crop production. When the individual is a plant, the family includes cultivars. The reference genome may be used to identify sperm (pollen) and eggs (stamen, pistil, ovule, and cone) for selective cultivation. For example, the reference genome may be used to predict or identify how plants will respond to pesticides, or environmental conditions such as drought, temperature, and atmosphere.

In still yet another embodiment, the reference genome is used to select microorganisms for research or industrial purposes. When the individual is a microorganism, the family includes strains within the same species or species, within the same genus. For example, the reference genome may be used to select antibiotics for which the microorganism is susceptible to. Alternatively, the reference genome may be used to select microorganisms for use in food or fuel production. For example, microorganisms with improved fermentative capabilities.

7.8 In Silico Genomics

In another aspect provided herein is a system comprising: (1) a central processing unit and (2) a memory coupled to the central processing unit, the memory storing one or more ancestral-specific reference genome databases provided herein. In certain embodiments, the memory further stores a nucleic acid comparison computer program wherein the nucleic acid sequencing computer program is capable of comparing the nucleic acid sequence of a sample nucleic acid with the plurality of ancestral-specific reference genomes of the one or more ancestral-specific reference genome databases to determine the presence of one or more ancestral-specific reference genome SNPs or haplotypes in the nucleic acid sequence of the sample nucleic acid sequence. In other embodiments, the system further comprises a user computer comprising an access software computer program that allows the access of the one or more ancestral-specific reference genome databases from the server computer. In yet other embodiments, the user computer further comprises a nucleic acid comparison computer program wherein the nucleic acid sequencing computer program is capable of comparing the nucleic acid sequence of a sample nucleic acid with the plurality of ancestral-specific reference genomes of the one or more ancestral-specific reference genome databases to determine the presence of one or more ancestral-specific reference genome SNPs or haplotypes in the nucleic acid sequence of the sample nucleic acid sequence.

The embodiments described herein are intended to be merely exemplary, and those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, numerous equivalents to the specific procedures described herein. All such equivalents are considered to be within the scope of the present invention and are covered by the following claims.

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All references (including patent applications, patents, and publications) cited herein are incorporated herein by reference in their entirety and for all purposes to the same extent as if each individual publication or patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety for all purposes. 

What is claimed is:
 1. A method for determining a subject's whole or partial ancestral-specific reference genome constructed by steps comprising: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; and (h) comparing a DNA sequence of the subject's whole or partial genome with the ancestral-specific reference genome to determine the whole or partial ancestral-specific genome of the subject.
 2. The method of claim 1, wherein the genome includes the epigenome.
 3. The method of claim 1, wherein the DNA sequence of the subject's genome is compared with the ancestral-specific reference genome with a nucleic acid comparison computer program.
 4. The method of claim 1, further comprising the step of recording the ancestral-specific reference genome database onto a tangible storage medium or a cloud-based storage solution.
 5. The method of claim 1, wherein the ancestral-specific reference genome is prepared by compiling the SNPs and/or haplotypes shared at a frequency of greater than 90%.
 6. The method of claim 1, further comprising the step of annotating the subject's genome based on an ancestral-specific reference database to determine disease-causing variants in the subject's genome.
 7. The method of claim 6, wherein the annotating step occurs before step a), at any point during steps a)-h), or after step h).
 8. The method of claim 1, wherein the ancestral-specific reference genome has 1×10⁶ or more SNPs.
 9. The method of claim 1, wherein the ancestral-specific reference genome has 3×10⁶ or more SNPs.
 10. The method of claim 1, wherein the subject is a human, animal, plant, or microorganism.
 11. The method of claim 1, wherein the DNA sequence of the subject's genome is compared with the ancestral-specific reference genome to identify disease-causing variants.
 12. A method of determining a subject's whole or partial ancestral-specific reference exome sequence constructed by the steps comprising: (a) obtaining a familial whole genome data set comprising whole genome DNA sequences from individuals of the subject's family; (b) comparing the whole genome DNA sequences within the familial whole genome data set to obtain a corrected familial whole genome data set; (c) preparing a first composite familial whole genome sequence from the corrected familial whole genome data set; (d) repeating steps a-c for a second, third or more families to obtain a second, third or more composite familial whole genome sequences; (e) evaluating the first, second, third or more composite familial whole genome sequences for single nucleotide polymorphisms (SNPs) and/or haplotypes and assigning statistical significance to the SNPs and/or haplotypes; (f) grouping the first, second, third or more composite familial whole genome sequences based on single nucleotide polymorphisms. (SNPs) and/or haplotypes that are statistically significant; (g) preparing the ancestral-specific reference genome by compiling, the SNPs and/or haplotypes shared by a group of composite familial whole genome sequences with the same ancestry; (h) comparing a DNA sequence of the subject's whole genome with the ancestral-specific reference whole genome to determine the coding regions of the subject's genome; and i) using the coding regions of the subject's genome identified to construct a whole or partial ancestral-specific exome sequence of the subject.
 13. The method of claim 12, wherein the subject is a human, animal, plant, or microorganism.
 14. The method of claim 12, further comprising the step of recording the ancestral-specific reference genome database onto a tangible storage medium or a cloud-based storage solution.
 15. The method of claim 12, wherein the ancestral-specific reference genome is prepared by compiling the SNPs and/or haplotypes shared at a frequency of greater than 90%.
 16. The method of claim 12, further comprising the step of annotating the subject's whole or partial exome based on an ancestral-specific reference database to determine disease-causing variants in the subject's exome.
 17. The method of claim 13, wherein the annotating step occurs before step a), at any point during steps a)-i), or after step i).
 18. A method for diagnosing a subject using an ancestral specific reference database comprising the steps of: (a) obtaining a subject's DNA; (b)sequencing the subject's DNA to assemble the subject's whole or partial genome or whole or partial exome; (c) during or prior to assembly of the subject's whole or partial genome or exome, annotating the subject's whole or partial genome or whole or partial exome based on the ancestral-specific reference database; and (d) identifying one or more genome sequence or exome sequence in the subject that differs from the one or more genome sequence or exome sequence in the ancestral-specific reference genome thereby identifying mutations or disease-causing variants in the one or more whole or partial genome sequence or whole or partial exome sequence of the subject's DNA to diagnose the subject.
 19. The method of claim 18, wherein the subject is diagnosed with a genetic disease selected from the group consisting of Achromatopsia, Aicardi Syndrome, Albinism, Alexander Disease, Alpers' Disease, Alzheimer's Disease, Angelman Syndrome, Autism, Bardet-Biedl Syndrome, Barth Syndrome, Best's Disease, Bipolar Disorder, Bloom Syndrome, Canavan Syndrome, Cancer, including Breast Cancer, Prostate Cancer, Ovarian Cancer, and other forms of cancer, including cancers resultant from germ-line and somatic mutations, Carnitine Deficiencies, Cerebral Palsy, Coffin Lowry Syndrome, Heart Defects, Hip Dysplasia, Cooley's Anemia, Corneal Dystrophy, Cystic Fibrosis, Cystinosis Diabetes, Down Syndrome, Epidermolysis Bullosa, Familial Dysautonomia, Fibrodysplasia, Fragile X Syndrome, Deficiency Anemia, Galactosemia, Gaucher Disease, Gilbert's Syndrome, Glaucoma, Hemochromatosis, Hemoglobin C Disease, Hemophilia/Bleeding Disorders, Hirschsprung's Disease, Homocystinuria, Huntington's Disease, Hurler Syndrome, Klinefelter Syndrome, Macular Degeneration, Marshall Syndrome, Menkes Disease, Metabolic Disorders, Microphthalmus, Mitochondrial Disease, Mucolipidoses, Muscular Dystrophy, Neonatal Onset Multisystem Inflammatory Disease, Neural Tube Defects, Noonan Syndrome, Optic Atrophy, Osteogenesis Imperfecta, Peutz-Jeghers Syndrome, Phenylketonuria (PKU), Pseudoxanthoma Elasticum, Progeria, Scheie Syndrome, Schizophrenia, Sickle Cell Anemia, Skeletal Dysplasias, Spherocytosis, Spina Bifida, Spinocerebellar Ataxia, Stargardt Disease (Macular Degeneration), Stickler Syndrome, Toy-Sachs Disease, Thalassemia, Treacher Collins Syndrome, Tuberous Sclerosis, Turner's Syndrome, Urea Cycle Disorder, Usher's Syndrome, and Werner Syndrome; Glycogen Branching. Enzyme Deficiency (GBED), Hereditary Equine Regional Dermal Asthenia (HERDA), Hyperkalemic Periodic Paralysis Disease (HYPP), Malignant Hyperthermia (MH), Polysaccharide Storage Myopathy—Type I (PSSMI), a-Mannosidosis, blood group incompatibility, neonatal isoerythrolysis, Burmese Head Defect, Deafness, Devon Rex Myopathy, Gangliosidosis, Glycogen storage disease type IV, Hypertrophic Cardiomyopathy, Hypertrophic Muscular dystrophy, Hypokalameic Polymyopathy, Manx Syndrome (spina bifida), Mucopolysaccharidosis, Niemann-Pick Disease, Osteochrondrodysplasia or Scottish Fold disease, Polycystic kidney disease, Polydactyl cats, Progressive retinal atrophy, Pyruvate kinase deficiency, Spinal muscular atrophy, Osteoarthritis, Hip dysplasia, Elbow dysplasia, Luxating patella, Osteochondritis dissecans (OCD), panosteitis, Legg-Calvé-Perthes syndrome, Congenital vertebral anomalies, Craniomandibular osteopathy, Spondylosis, Masticatory muscle myosis, von Wilebrand disease, Thrombocytopenia, Thromboystosis, Hemolytic anemia, Tetra-logy of Fallot, Patent ductus arteriosis, Heart valve dysplasia, Cor triatriatum, Subvalvular Aortic stenosis, Pulmonic stenosis, Ventricular septal defect, Atrial septal defect, Epilepsy, Eyelid disease, Retinal disease, Lens disease, Corneal disease, and Endocrine Disease.
 20. The method of claim 18, wherein the subject is a human, animal, plant, or microorganism. 